Since 1970

ABOUT US

Irvine Scientific, a member of FUJIFILM Corporation, is a worldwide leader in the innovation and manufacture of cell culture media, reagents, and medical devices for researchers and clinicians. The company provides unrivalled service and quality to scientists working in cell therapy and regenerative medicine, assisted reproductive technology and cytogenetics, and industrial cell culture for the large-scale production of biotherapeutics and vaccines. Irvine Scientific adheres to both ISO and FDA regulations and operates dual cGMP manufacturing facilities in California, USA and Tokyo, Japan. The company’s consultative philosophy combined with expertise in cell culture and compliance provides customers with unique capabilities and support. For over 40 years Irvine Scientific has remained uniquely flexible and focused on media while becoming a strategic global leader in media products and services.

PBS 1X - DULBECCO'S PHOSPHATE BUFFERED SALINE SOLUTION

Catalog ID : 9235

500ml

PBS 1X - Dulbecco's Phosphate Buffered Saline Solution is a synthetic, defined solution for procedures which include the retrieval, handling, and transfer of human gametes and embryos.

Liquid, Ready-To-Use

Without:

Sodium Bicarbonate
Phenol Red
Protein
Mouse Embryo Tested

Standard Packaging Size: 500 mL

HAM'S F-10 - LIQUID

Catalog ID : 9056

500ml

Ham's F-10 was originally developed for use in cloning Chinese hamster ovary and lung cells. This medium is useful with serum supplements for a variety of cell culture applications, including the growth of myeloma and hybridoma cells.

With:

  • 1200 mg/L Sodium Bicarbonate

 

Without:

MULTIPURPOSE HANDLING MEDIUM® -COMPLETE (MHM®-C) WITH GENTAMICIN

Catalog ID : 90166

100ml, 500ml

Handle sperm, oocytes, and embryos in a stable environment

Multipurpose Handling Medium® (MHM®) is dual buffered solution (HEPES and MOPS) that provides a safe and secure environment to maintain viability of gametes and embryos during manipulations under ambient conditions. It is a versatile solution for swim up preparation, sperm washing, oocyte retrieval and rinsing, IUI, ICSI, and embryo transfer.

  • Maintain physiological pH 7.2-7.4 and osmolality over a broad temperature range

  • Support maintenance of cellular homeostasis with aid of glycine and taurine

  • Reduce risk of toxicity using a dual buffer system

  • Save time - no mixing or filtering, ready to use

 

Also available in:
90163 - Multipurpose Handling Medium (MHM)

MODIFIED HAM'S F-10 BASAL MEDIUM - HEPES

Catalog ID : 99168

100ml

Modified Ham’s F-10 Basal Media are intended for use in assisted reproductive procedures which include gamete and embryo manipulation. Supplementation with additional components (sodium lactate, sodium pyruvate, glutamine and gentamicin) are required prior to use. Modified Ham’s F-10 - HEPES (Catalog #99168) has been specifically formulated without hypoxanthine, and with HEPES for buffering at ambient atmosphere, and does not require the use of a CO2incubator. 100 mL.

Liquid, basal medium

With

  • Bicarbonate

  • HEPES buffer

  • Phenol Red

Without

  • Protein

  • Antibiotics

  • Hypoxanthine

MODIFIED HTF MEDIUM WITH GENTAMICIN - HEPES

Catalog ID : 90126​

100ml, 500ml

For handling and transfer of human gametes and embryos

Modified HTF Medium is a synthetic, defined solution for procedures which include the retrieval, handling and transfer of human gametes and embryos. mHTF is composed of a HEPES and sodium bicarbonate buffering system, making it ideal for use in ambient atmosphere conditions. It requires protein supplementation before use.

HTF MEDIUM WITH GENTAMICIN

Catalog ID : 90125

100ml, 500ml(*)

 

 

HTF Media are intended for use in assisted reproductive procedures which include gamete and embryo manipulation through Day 3 of development. HTF Medium is a synthetic, defined solution for use as a culture media through Day 3 of human embryo development as well as the processing of gametes. HTF is bicarbonate-based and is designed for use in a CO2 incubator.

Requires protein supplementation 100 mL

P-1® MEDIUM WITH GENTAMICIN

Catalog ID : 99242

100ml

P-1® Medium is intended for use in culturing human gametes and embryos during fertilization and growth through Day 3 of development. P-1® Medium was developed by Dr. Thomas B. Pool, Fertility Center of San Antonio, based upon the original HTF formula but modified to provide a glucose-free and phosphate-free environment. This formulation also contains taurine, an amino acid shown to have protective and embryogenic functions. P-1® Medium is bicarbonate-based and is designed for procedures utilizing a CO2 incubator.

Requires Protein Supplementation. 100 mL.

EARLY CLEAVAGE MEDIUM® (ECM®)

Catalog ID : 90138

60ml

Early Cleavage Medium® (ECM®) is intended for use in culturing human gametes during fertilization (IVF) and growth of embryos through Day 3 of development. ECM® is a modification of P-1® Medium that contains glucose, alanyl-glutamine and EDTA, components shown to benefit human embryo development. ECM® is bicarbonate based and is designed for procedures utilizing a CO2 incubator.

Requires protein supplementation.

60 mL.

Also available in:
90142 - ECM® with DSS

MULTIBLAST MEDIUM®

Catalog ID : 90139

60ml

MultiBlast Medium® is intended for use in culturing human embryos from Day 3 to blastocyst (up to Day 6). MultiBlast Medium® is formulated to provide a complex array of components required for ongoing development of embryos to the blastocyst stage. MultiBlast Medium® is bicarbonate based and is designed for use in a CO2 incubator.

Requires protein supplementation.

60 mL.

Also available in:
90140 - ECM® with SSS™
90142 - ECM® with DSS

Complete Early Cleavage Medium® (ECM®) with DSS

Catalog ID : 90142

2x20ml

Complete Early Cleavage Medium® (ECM®) is intended for use in culturing human gametes during fertilization (IVF) and growth of embryos through Day 3 of development. ECM® is a modification of P-1® Medium that contains glucose, alanyl-glutamine and EDTA, components shown to benefit human embryo development. ECM® is bicarbonate based and is designed for procedures utilizing a CO2 incubator.

Available in 2 x 20 mL configuration. Already supplemented with protein (10% DSS) for a final total macromolecule concentration of 7 mg/mL.

Also available in:
90138 - ECM®

Cmplete MultiBlast Medium® with DSS

Catalog ID : 90143

2x20ml

Complete MultiBlast Medium® is intended for use in culturing human embryos from Day 3 to blastocyst (up to Day 6). MultiBlast Medium® is formulated to provide a complex array of components required for ongoing development of embryos to the blastocyst stage. MultiBlast Medium® is bicarbonate based and is designed for use in a CO2 incubator.

Available in 2 x 20 mL configuration. Already supplemented with protein (10% DSS) for a final total macromolecule concentration of 7 mg/mL.

Also available in:


90139 - MultiBlast Medium®

 

CONTINUOUS SINGLE CULTURE

Cat. No. 90164

60ml

 

Continuous Single Culture® product (CSCM) is a novel solution designed for fertilization and embryo culture through day 5/6 of embryo development. CSCM is optimized to be used in an uninterrupted culture system without dish change or medium renewal.

CSCM is bicarbonate-based and is designed for use in a CO2 incubator.

Requires protein supplementation.

60 mL.

Also available in:


90165 - Continuous Single Culture® Complete with Human Serum Albumin

             

NEW!   

Continuous Single Culture® -NX Complete With HSA

Cat. No. 90168

2X20ml

 

Clinically proven, low lactate, single-step culture medium that helps maintain efficient metabolic rates by eliminating unnecessary stress.

Continuous Single Culture® -NX Complete (CSCM -NXC) is a clinically-proven, low lactate, single-step culture medium that helps improves blastocyst development. CSCM -NXC is designed for fertilization and embryo culture through day 5/6 of embryo development. This medium is optimized to be used in an uninterrupted culture system, without dish change or medium renewal.

Pre-supplemented with HSA for a final total protein concentration of 5 mg/mL.

Available in 2x20mL configuration.

Also available in:


90167 - Continuous Single Culture®- NX

Human Serum Albumin Solution

Cat. No. 9988

12x5ml, 100ml

 

Human Serum Albumin consists of HSA (100 mg/ml) from a therapeutic grade source material in a saline solution. This represents 10% total protein (weight/volume), in normal saline.

Available in 12x5 mL and 100 mL.

Serum Substitute Supplement™

Cat. No. 99193

12x5ml, 100ml

 

Serum Substitute Supplement™ (SSS™) contains 6% (w/v) total protein, in normal saline.  This consists of 84% HSA (50mg/mL, 5% w/v) from a therapeutic grade source material and 16% alpha and beta Globulins (10 mg/mL, 1% w/v) in a saline solution.

Available in 12 x 12 mL & 100 mL.

Dextran Serum Supplement

Cat. No. 9301

12x12ml, 100ml

 

Dextran Serum Supplement consists of 100% HSA (50 mg/mL, 5% w/v) from a therapeutic grade source material and dextran (20 mg/mL, 2% w/v) added as a non-protein alternative to globulins, in a saline solution.

5% total protein (weight/volume), in normal saline.

Available in 100 mL and 12x12 mL packaging

ISolate®

Cat. No. 99264

2x50ml

 

Sperm separation kit designed to quickly produce clean samples, with high concentrations of progressive motile sperm from both healthy and low quality semen.

As one of the most cited density gradient sperm separation media, ISolate is the media of choice for laboratories worldwide to produce clean, robust samples with optimal recovery rates. It is supplied ready-to-use as a dual density gradient solution to process the wide variety of semen samples encountered in the laboratory. The kit, which consists of a 50% upper layer and 90% lower layer, support use with a two layer protocol for healthy samples or with a single layer protocol that enables separation of motile sperm from low quality semen samples. ISolate effectively removes cellular contaminants (dead sperm, white cells, and miscellaneous debris) to yield a sample that contains predominantly progressive, motile sperm, capable of fertilizing the oocyte for IUI, IVF and ICSI.

• Formulated with sterile, colloidal suspension of uniform silica particles stabilized with covalently-bound hydrophilic silane in HEPES-buffered HTF
• Highest quality raw materials
• Quality tested by human sperm survival assay
• 90% stock and 100% concentrate available for custom dilutions
• CE Marked
 

Quality Control Testing:

• Rabbit pyrogen test
• Sterility by the current USP, CFR or Ph. Eur. Sterility Test
• Sperm Survival Assay
• pH
• Osmolality
• Endotoxin by Limulus Amebocyte Lysate (LAL)

ISolate® Stock Solution

Cat. No. 99275

100ml

 

Single 90% gradient solution for rapid single layer separation of sperm or for creating custom two-layer gradient systems.

ISolate Stock is a 90% density gradient medium used for single layer sperm separation or as the lower layer of a customized gradient developed by the end user based on the semen quality. As one of the most cited density gradient sperm separation media, ISolate Stock is the ideal medium for laboratories that prefer single layer separation or more control over their sperm yields.

• Formulated with sterile, colloidal suspension of uniform silica particles stabilized with covalently-bound hydrophilic silane in HEPES-buffered HTF
• Quality tested by human sperm survival assay
• ISolate can be diluted with mHTF or Multipurpose Handling Medium (MHM)
• Supplied in large economical packages
• CE Marked

Quality Control Testing:
• Rabbit pyrogen test
• Sterility by the current USP, CFR or Ph. Eur. Sterility Test
• Sperm Survival Assay
• pH
• Osmolality

ISolate® Concentrate

Cat. No. 99306

100ml

 

Prepare custom density gradients to isolate desired quantities of motile sperm.

ISolate Concentrate (100%) provides full control over the density gradient sperm separation process. Large volume packaging allows for economical development of custom gradients that meet your laboratory’s procedures and needs. As one of the most cited density gradient sperm separation media, ISolate Concentrate is the ideal product for customizable sperm separation.

• ISolate can be diluted with mHTF or Multipurpose Handling Medium (MHM)
• Formulated with sterile, colloidal suspension of uniform silica particles stabilized with covalently-bound hydrophilic silane in HEPES-buffered HTF
• Quality tested by human sperm survival assay
• CE Marked

Quality Control Testing:

• Rabbit pyrogen test
• Sterility by the current USP, CFR and Ph. Eur.  Sterility Test
• Sperm Survival Assay
• pH
• Osmolality
• Endotoxin by Limulus Amebocyte Lysate (LAL)

Sperm Washing Medium

Cat. No. 9983

100ml

 

Developed to maintain sperm quality during in vitro washing procedures and sperm preparation outside the incubator.

Sperm Washing Medium supports all steps of sperm preparation prior to fertilization, whether it is IUI, IVF, or other ART procedures. It is designed to maintain sperm quality on the tabletop without CO2 incubation. Sperm Washing Medium provides lot-to-lot  consistency for sperm processing through its quality formulation, which is stringently tested by mouse embryo assay (MEA).


• Formulation: HEPES-buffered HTF with human serum albumin
• Does not contain antibiotics
• CE Marked

Quality Control Testing:
• Endotoxin by Limulus Amebocyte Lysate (LAL)
• Mouse Embryo Assay
• Sterility by the current USP, CFR or Ph. Eur. Sterility Test
• pH
• Osmolality

Hyaluronidase Solution

Cat. No. 90101

5x1ml

 

For the removal of cumulus cells surrounding freshly retrieved oocytes.

Hyaluronidase Solution cleans fresh oocytes prior to ICSI or other ART procedures. Each lot of hyaluronidase solution is tested for an enzymatic activity of 80 IU/mL to facilitate consistent removal of the cumulus cells surrounding retrieved oocytes.

• CE Marked
• Contains:
   - 80 IU/mL of hyaluronidase enzyme in HEPES-buffered HTF
   - Human serum albumin (5 mg/mL)
   - Gentamicin sulfate (10 µg/mL)

Polyvinylpyrrolidone (PVP) Solution with HSA - 7%

Cat. No. 90121

5x0.5ml

 

Gain greater control over sperm selection for easier micromanipulation of low quality or low motility sperm in ICSI procedures.

Polyvinylpyrrolidone (PVP) with HSA - 7% is a viscous solution that allows for easier sperm micromanipulation by slowing or immobilizing sperm for intracytoplasmic sperm injection (ICSI). PVP 7% has relatively less viscosity than PVP 10% and is recommended for use with low quality or low motility sperm. PVP solution also yields greater control over fluid flow and prevents sticking in the ICSI pipette.

• Contains human serum albumin (5 mg/mL) and reconstituted in isotonic HEPES-buffered HTF medium.
• CE Marked

Quality Control Testing:
• Endotoxin by Limulus Amebocyte Lysate (LAL)
• Sterility by the current USP, CFR and Ph. Eur. Sterility Test
• Sperm Motility Recovery Assay

Polyvinylpyrrolidone (PVP) Solution with HSA - 10%

Cat. No. 90123

5x0.5ml

 

Decrease the motility of sperm for easier micromanipulation of high motility and healthy sperm in ICSI procedures

Polyvinylpyrrolidone (PVP) 10% is a viscous solution that allows for easier sperm micromanipulation by slowing or decreasing the motility of sperm for intracytoplasmic sperm injection (ICSI). PVP 10% has relatively more viscosity than PVP 7% and is recommended for use with high motility and healthy sperm. PVP solution yields greater control over fluid flow and prevents sticking in the ICSI pipette.

• Contains human serum albumin (5 mg/mL) and reconstituted in isotonic HEPES-buffered HTF medium.
• CE Marked

Quality Control Testing:
• Endotoxin by Limulus Amebocyte Lysate (LAL)
• Sterility by the current USP, CFR and Ph. Eur.  Sterility Test
• Sperm Motility Recovery Assay

Polyvinylpyrrolidone (PVP) - Lyophilized

Cat. No. 99219

10x1ml

 

Develop custom PVP concentrations for desired Immobilization and micromanipulation of sperm in ICSI procedures.

Polyvinylpyrrolidone (PVP) - Lyophilized is intended for use in assisted reproductive procedures that include gamete and embryo manipulation. These procedures include the use of PVP solution for immobilizing sperm for ICSI procedures. PVP - Lyophilized is a powder form which requires aseptic reconstitution with mHTF or other appropriate media prior to use. 

As a powder, PVP - Lyophilized has a 2 year shelf life and allows for custom PVP concentrations to suit your laboratory processes. Once reconstituted, lyophilized PVP may be stored for up to 3 weeks. This product does not contain protein supplement.

Quality Control Testing:
• Endotoxin by Limulus Amebocyte Lysate (LAL)
• Sterility by the current USP, CFR and Ph. Eur. Sterility Test
• Sperm Motility Recovery Assay

Also available in:

Tyrode's Solution - Acidified

Cat. No. 99252

12x5ml

 

High acidity solution for drilling the zona pellucida during assisted hatching or biopsy procedures​

Tyrode's Solution - Acidified facilitates assisted hatching by chemically drilling the zona pellucida. With a pH of 2.1-2.5, this solution's high acidity can break the zona pellucida while avoiding damage to the embryo if applied carefully and in small quantities.

Quality Control Testing:
• Endotoxin by Limulus Amebocyte Lysate (LAL)
• Sterility by the current USP, CFR or Ph. Eur. Sterility Test
• pH
• Osmolality

Embryo Biopsy Medium

Cat. No. 90103

2x20ml

 

Remove blastomeres for pre-implantation genetic diagnosis (PGD) from Day 3 cleavage-stage embryos

Embryo Biopsy Medium is for temporary culture and holding of day 3 embryos during biopsy. It is an ideal environment for 4-10 cell embryos that will have blastomeres removed for pre-implantation genetic screening or diagnosis.

Contains:

  • HEPES-buffered HTF without Ca2+ and Mg2+

  • EDTA as chelating agent

  • Human serum albumin (5 mg/mL)

  • Gentamicin sulfate (10µg/mL)

Oil for Embryo Culture

Cat. No. 9305

100, 500ml

 

Oil for Embryo Culture is a sterile light mineral oil intended for use as an overlay when culturing in reduced volumes of media to prevent evaporation, and to protect the media from changes in osmolality and pH.

Standard Packaging Size: 100 mL, 500 mL

Vit Kit®-Freeze

Cat. No. 90133-SO

 

Vitrification Freeze Kit is for use in the vitrification of oocytes (MII), pronuclear (PN) zygotes through day 3 cleavage stage embryos and blastocyst stage embryos.

Each kit contains liquid and ready-to-use solutions. It does not require CO2 incubation.

2 Vials of ES (Equilibration Solution, 2 x 1 mL). Contains

  • 7.5% DMSO

  • 7.5% ethylene glycol

  • 20% DSS

  • Gentamicin

  • In a M-199 HEPES Buffered Medium

 

2 Vials of VS (Vitrification Solution, 2 x 1 mL). Contains

  • 15% DMSO

  • 15% ethylene glycol

  • 20% DSS

  • 0.5 M sucrose

  • Gentamicin

  • In a M-199 HEPES Buffered Medium

Vit Kit®-Thaw

Cat. No. 90137-SO

 

Vitrification Thaw Kit is for use in the thawing of vitrified oocytes (MII), pronuclear (PN) zygotes through day 3 cleavage stage embryos and blastocyst stage embryos.

Vitrification Thaw Kit is for use in the thawing of vitrified oocytes (MII), pronuclear (PN) zygotes through day 3 cleavage stage embryos and blastocyst stage embryos.

Each kit contains liquid and ready-to-use solutions. It does not require CO2 incubation.

4 Vials of TS (Thawing Solution, 4 x 2mL). Contains

  • 1 M sucrose

  • 20% DSS

  • Gentamicin

  • In a M-199 HEPES Buffered Medium

 

1 Vial of DS (Dilution Solution, 1 x 2mL). Contains

  • 0.5 M sucrose

  • 20% DSS

  • Gentamicin

  • In a M-199 HEPES Buffered Medium

 

1 Vial of WS (Washing Solution, 1 x 2mL). Contains

  • 20% DSS

  • Gentamicin

  • In a M-199 HEPES Buffered Medium.