Since 1970
ABOUT US
Irvine Scientific, a member of FUJIFILM Corporation, is a worldwide leader in the innovation and manufacture of cell culture media, reagents, and medical devices for researchers and clinicians. The company provides unrivalled service and quality to scientists working in cell therapy and regenerative medicine, assisted reproductive technology and cytogenetics, and industrial cell culture for the large-scale production of biotherapeutics and vaccines. Irvine Scientific adheres to both ISO and FDA regulations and operates dual cGMP manufacturing facilities in California, USA and Tokyo, Japan. The company’s consultative philosophy combined with expertise in cell culture and compliance provides customers with unique capabilities and support. For over 40 years Irvine Scientific has remained uniquely flexible and focused on media while becoming a strategic global leader in media products and services.
PBS 1X - DULBECCO'S PHOSPHATE BUFFERED SALINE SOLUTION
Catalog ID : 9235
500ml
PBS 1X - Dulbecco's Phosphate Buffered Saline Solution is a synthetic, defined solution for procedures which include the retrieval, handling, and transfer of human gametes and embryos.
Liquid, Ready-To-Use
Without:
Sodium Bicarbonate
Phenol Red
Protein
Mouse Embryo Tested
Standard Packaging Size: 500 mL
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Product Inserts
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SDS
HAM'S F-10 - LIQUID
Catalog ID : 9056
500ml
Ham's F-10 was originally developed for use in cloning Chinese hamster ovary and lung cells. This medium is useful with serum supplements for a variety of cell culture applications, including the growth of myeloma and hybridoma cells.
With:
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1200 mg/L Sodium Bicarbonate
Without:
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L-Glutamine - add 5 mL/L (200 mM)
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SDS
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Brochures
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Formulations
MULTIPURPOSE HANDLING MEDIUM® -COMPLETE (MHM®-C) WITH GENTAMICIN
Catalog ID : 90166
100ml, 500ml
Handle sperm, oocytes, and embryos in a stable environment
Multipurpose Handling Medium® (MHM®) is dual buffered solution (HEPES and MOPS) that provides a safe and secure environment to maintain viability of gametes and embryos during manipulations under ambient conditions. It is a versatile solution for swim up preparation, sperm washing, oocyte retrieval and rinsing, IUI, ICSI, and embryo transfer.
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Maintain physiological pH 7.2-7.4 and osmolality over a broad temperature range
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Support maintenance of cellular homeostasis with aid of glycine and taurine
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Reduce risk of toxicity using a dual buffer system
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Save time - no mixing or filtering, ready to use
Also available in:
90163 - Multipurpose Handling Medium (MHM)
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Product Inserts
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Product Sheets
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White Papers and Research Articles
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Brochures
MODIFIED HAM'S F-10 BASAL MEDIUM - HEPES
Catalog ID : 99168
100ml
Modified Ham’s F-10 Basal Media are intended for use in assisted reproductive procedures which include gamete and embryo manipulation. Supplementation with additional components (sodium lactate, sodium pyruvate, glutamine and gentamicin) are required prior to use. Modified Ham’s F-10 - HEPES (Catalog #99168) has been specifically formulated without hypoxanthine, and with HEPES for buffering at ambient atmosphere, and does not require the use of a CO2incubator. 100 mL.
Liquid, basal medium
With
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Bicarbonate
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HEPES buffer
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Phenol Red
Without
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Protein
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Antibiotics
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Hypoxanthine
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Product Sheets
MODIFIED HTF MEDIUM WITH GENTAMICIN - HEPES
Catalog ID : 90126
100ml, 500ml
For handling and transfer of human gametes and embryos
Modified HTF Medium is a synthetic, defined solution for procedures which include the retrieval, handling and transfer of human gametes and embryos. mHTF is composed of a HEPES and sodium bicarbonate buffering system, making it ideal for use in ambient atmosphere conditions. It requires protein supplementation before use.
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HTF MEDIUM WITH GENTAMICIN
Catalog ID : 90125
100ml, 500ml(*)
HTF Media are intended for use in assisted reproductive procedures which include gamete and embryo manipulation through Day 3 of development. HTF Medium is a synthetic, defined solution for use as a culture media through Day 3 of human embryo development as well as the processing of gametes. HTF is bicarbonate-based and is designed for use in a CO2 incubator.
Requires protein supplementation 100 mL
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Production Schedule
P-1® MEDIUM WITH GENTAMICIN
Catalog ID : 99242
100ml
P-1® Medium is intended for use in culturing human gametes and embryos during fertilization and growth through Day 3 of development. P-1® Medium was developed by Dr. Thomas B. Pool, Fertility Center of San Antonio, based upon the original HTF formula but modified to provide a glucose-free and phosphate-free environment. This formulation also contains taurine, an amino acid shown to have protective and embryogenic functions. P-1® Medium is bicarbonate-based and is designed for procedures utilizing a CO2 incubator.
Requires Protein Supplementation. 100 mL.
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Production Schedule
EARLY CLEAVAGE MEDIUM® (ECM®)
Catalog ID : 90138
60ml
Early Cleavage Medium® (ECM®) is intended for use in culturing human gametes during fertilization (IVF) and growth of embryos through Day 3 of development. ECM® is a modification of P-1® Medium that contains glucose, alanyl-glutamine and EDTA, components shown to benefit human embryo development. ECM® is bicarbonate based and is designed for procedures utilizing a CO2 incubator.
Requires protein supplementation.
60 mL.
Also available in:
90142 - ECM® with DSS
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Product Inserts
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SDS
MULTIBLAST MEDIUM®
Catalog ID : 90139
60ml
MultiBlast Medium® is intended for use in culturing human embryos from Day 3 to blastocyst (up to Day 6). MultiBlast Medium® is formulated to provide a complex array of components required for ongoing development of embryos to the blastocyst stage. MultiBlast Medium® is bicarbonate based and is designed for use in a CO2 incubator.
Requires protein supplementation.
60 mL.
Also available in:
90140 - ECM® with SSS™
90142 - ECM® with DSS
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SDS
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Product Sheets
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Production Schedule
Complete Early Cleavage Medium® (ECM®) with DSS
Catalog ID : 90142
2x20ml
Complete Early Cleavage Medium® (ECM®) is intended for use in culturing human gametes during fertilization (IVF) and growth of embryos through Day 3 of development. ECM® is a modification of P-1® Medium that contains glucose, alanyl-glutamine and EDTA, components shown to benefit human embryo development. ECM® is bicarbonate based and is designed for procedures utilizing a CO2 incubator.
Available in 2 x 20 mL configuration. Already supplemented with protein (10% DSS) for a final total macromolecule concentration of 7 mg/mL.
Also available in:
90138 - ECM®
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Product Inserts
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SDS
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Production Schedule
Cmplete MultiBlast Medium® with DSS
Catalog ID : 90143
2x20ml
Complete MultiBlast Medium® is intended for use in culturing human embryos from Day 3 to blastocyst (up to Day 6). MultiBlast Medium® is formulated to provide a complex array of components required for ongoing development of embryos to the blastocyst stage. MultiBlast Medium® is bicarbonate based and is designed for use in a CO2 incubator.
Available in 2 x 20 mL configuration. Already supplemented with protein (10% DSS) for a final total macromolecule concentration of 7 mg/mL.
Also available in:
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Production Schedule
CONTINUOUS SINGLE CULTURE
Cat. No. 90164
60ml
Continuous Single Culture® product (CSCM) is a novel solution designed for fertilization and embryo culture through day 5/6 of embryo development. CSCM is optimized to be used in an uninterrupted culture system without dish change or medium renewal.
CSCM is bicarbonate-based and is designed for use in a CO2 incubator.
Requires protein supplementation.
60 mL.
Also available in:
90165 - Continuous Single Culture® Complete with Human Serum Albumin
NEW!
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Protocols
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White Papers and Research Articles
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Scientific Posters
Continuous Single Culture® -NX Complete With HSA
Cat. No. 90168
2X20ml
Clinically proven, low lactate, single-step culture medium that helps maintain efficient metabolic rates by eliminating unnecessary stress.
Continuous Single Culture® -NX Complete (CSCM -NXC) is a clinically-proven, low lactate, single-step culture medium that helps improves blastocyst development. CSCM -NXC is designed for fertilization and embryo culture through day 5/6 of embryo development. This medium is optimized to be used in an uninterrupted culture system, without dish change or medium renewal.
Pre-supplemented with HSA for a final total protein concentration of 5 mg/mL.
Available in 2x20mL configuration.
Also available in:
90167 - Continuous Single Culture®- NX
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Product Inserts
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Protocols
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Human Serum Albumin Solution
Cat. No. 9988
12x5ml, 100ml
Human Serum Albumin consists of HSA (100 mg/ml) from a therapeutic grade source material in a saline solution. This represents 10% total protein (weight/volume), in normal saline.
Available in 12x5 mL and 100 mL.
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Serum Substitute Supplement™
Cat. No. 99193
12x5ml, 100ml
Serum Substitute Supplement™ (SSS™) contains 6% (w/v) total protein, in normal saline. This consists of 84% HSA (50mg/mL, 5% w/v) from a therapeutic grade source material and 16% alpha and beta Globulins (10 mg/mL, 1% w/v) in a saline solution.
Available in 12 x 12 mL & 100 mL.
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Dextran Serum Supplement
Cat. No. 9301
12x12ml, 100ml
Dextran Serum Supplement consists of 100% HSA (50 mg/mL, 5% w/v) from a therapeutic grade source material and dextran (20 mg/mL, 2% w/v) added as a non-protein alternative to globulins, in a saline solution.
5% total protein (weight/volume), in normal saline.
Available in 100 mL and 12x12 mL packaging
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Product Inserts
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SDS
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Product Sheets
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Brochures
ISolate®
Cat. No. 99264
2x50ml
Sperm separation kit designed to quickly produce clean samples, with high concentrations of progressive motile sperm from both healthy and low quality semen.
As one of the most cited density gradient sperm separation media, ISolate is the media of choice for laboratories worldwide to produce clean, robust samples with optimal recovery rates. It is supplied ready-to-use as a dual density gradient solution to process the wide variety of semen samples encountered in the laboratory. The kit, which consists of a 50% upper layer and 90% lower layer, support use with a two layer protocol for healthy samples or with a single layer protocol that enables separation of motile sperm from low quality semen samples. ISolate effectively removes cellular contaminants (dead sperm, white cells, and miscellaneous debris) to yield a sample that contains predominantly progressive, motile sperm, capable of fertilizing the oocyte for IUI, IVF and ICSI.
• Formulated with sterile, colloidal suspension of uniform silica particles stabilized with covalently-bound hydrophilic silane in HEPES-buffered HTF
• Highest quality raw materials
• Quality tested by human sperm survival assay
• 90% stock and 100% concentrate available for custom dilutions
• CE Marked
Quality Control Testing:
• Rabbit pyrogen test
• Sterility by the current USP, CFR or Ph. Eur. Sterility Test
• Sperm Survival Assay
• pH
• Osmolality
• Endotoxin by Limulus Amebocyte Lysate (LAL)
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Product Inserts
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SDS
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Product Sheets
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Protocols
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White Papers and Research Articles
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Brochures
ISolate® Stock Solution
Cat. No. 99275
100ml
Single 90% gradient solution for rapid single layer separation of sperm or for creating custom two-layer gradient systems.
ISolate Stock is a 90% density gradient medium used for single layer sperm separation or as the lower layer of a customized gradient developed by the end user based on the semen quality. As one of the most cited density gradient sperm separation media, ISolate Stock is the ideal medium for laboratories that prefer single layer separation or more control over their sperm yields.
• Formulated with sterile, colloidal suspension of uniform silica particles stabilized with covalently-bound hydrophilic silane in HEPES-buffered HTF
• Quality tested by human sperm survival assay
• ISolate can be diluted with mHTF or Multipurpose Handling Medium (MHM)
• Supplied in large economical packages
• CE Marked
Quality Control Testing:
• Rabbit pyrogen test
• Sterility by the current USP, CFR or Ph. Eur. Sterility Test
• Sperm Survival Assay
• pH
• Osmolality
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Product Inserts
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SDS
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Product Sheets
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Brochures
ISolate® Concentrate
Cat. No. 99306
100ml
Prepare custom density gradients to isolate desired quantities of motile sperm.
ISolate Concentrate (100%) provides full control over the density gradient sperm separation process. Large volume packaging allows for economical development of custom gradients that meet your laboratory’s procedures and needs. As one of the most cited density gradient sperm separation media, ISolate Concentrate is the ideal product for customizable sperm separation.
• ISolate can be diluted with mHTF or Multipurpose Handling Medium (MHM)
• Formulated with sterile, colloidal suspension of uniform silica particles stabilized with covalently-bound hydrophilic silane in HEPES-buffered HTF
• Quality tested by human sperm survival assay
• CE Marked
Quality Control Testing:
• Rabbit pyrogen test
• Sterility by the current USP, CFR and Ph. Eur. Sterility Test
• Sperm Survival Assay
• pH
• Osmolality
• Endotoxin by Limulus Amebocyte Lysate (LAL)
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Product Inserts
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SDS
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Product Sheets
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Brochures
Sperm Washing Medium
Cat. No. 9983
100ml
Developed to maintain sperm quality during in vitro washing procedures and sperm preparation outside the incubator.
Sperm Washing Medium supports all steps of sperm preparation prior to fertilization, whether it is IUI, IVF, or other ART procedures. It is designed to maintain sperm quality on the tabletop without CO2 incubation. Sperm Washing Medium provides lot-to-lot consistency for sperm processing through its quality formulation, which is stringently tested by mouse embryo assay (MEA).
• Formulation: HEPES-buffered HTF with human serum albumin
• Does not contain antibiotics
• CE Marked
Quality Control Testing:
• Endotoxin by Limulus Amebocyte Lysate (LAL)
• Mouse Embryo Assay
• Sterility by the current USP, CFR or Ph. Eur. Sterility Test
• pH
• Osmolality
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Product Inserts
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SDS
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Product Sheets
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Sperm Washing Media
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Protocols
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Brochures
Hyaluronidase Solution
Cat. No. 90101
5x1ml
For the removal of cumulus cells surrounding freshly retrieved oocytes.
Hyaluronidase Solution cleans fresh oocytes prior to ICSI or other ART procedures. Each lot of hyaluronidase solution is tested for an enzymatic activity of 80 IU/mL to facilitate consistent removal of the cumulus cells surrounding retrieved oocytes.
• CE Marked
• Contains:
- 80 IU/mL of hyaluronidase enzyme in HEPES-buffered HTF
- Human serum albumin (5 mg/mL)
- Gentamicin sulfate (10 µg/mL)
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Product Inserts
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SDS
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Product Sheets
Polyvinylpyrrolidone (PVP) Solution with HSA - 7%
Cat. No. 90121
5x0.5ml
Gain greater control over sperm selection for easier micromanipulation of low quality or low motility sperm in ICSI procedures.
Polyvinylpyrrolidone (PVP) with HSA - 7% is a viscous solution that allows for easier sperm micromanipulation by slowing or immobilizing sperm for intracytoplasmic sperm injection (ICSI). PVP 7% has relatively less viscosity than PVP 10% and is recommended for use with low quality or low motility sperm. PVP solution also yields greater control over fluid flow and prevents sticking in the ICSI pipette.
• Contains human serum albumin (5 mg/mL) and reconstituted in isotonic HEPES-buffered HTF medium.
• CE Marked
Quality Control Testing:
• Endotoxin by Limulus Amebocyte Lysate (LAL)
• Sterility by the current USP, CFR and Ph. Eur. Sterility Test
• Sperm Motility Recovery Assay
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Product Inserts
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SDS
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Product Sheets
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Brochures
Polyvinylpyrrolidone (PVP) Solution with HSA - 10%
Cat. No. 90123
5x0.5ml
Decrease the motility of sperm for easier micromanipulation of high motility and healthy sperm in ICSI procedures
Polyvinylpyrrolidone (PVP) 10% is a viscous solution that allows for easier sperm micromanipulation by slowing or decreasing the motility of sperm for intracytoplasmic sperm injection (ICSI). PVP 10% has relatively more viscosity than PVP 7% and is recommended for use with high motility and healthy sperm. PVP solution yields greater control over fluid flow and prevents sticking in the ICSI pipette.
• Contains human serum albumin (5 mg/mL) and reconstituted in isotonic HEPES-buffered HTF medium.
• CE Marked
Quality Control Testing:
• Endotoxin by Limulus Amebocyte Lysate (LAL)
• Sterility by the current USP, CFR and Ph. Eur. Sterility Test
• Sperm Motility Recovery Assay
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Product Inserts
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SDS
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Product Sheets
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Brochures
Polyvinylpyrrolidone (PVP) - Lyophilized
Cat. No. 99219
10x1ml
Develop custom PVP concentrations for desired Immobilization and micromanipulation of sperm in ICSI procedures.
Polyvinylpyrrolidone (PVP) - Lyophilized is intended for use in assisted reproductive procedures that include gamete and embryo manipulation. These procedures include the use of PVP solution for immobilizing sperm for ICSI procedures. PVP - Lyophilized is a powder form which requires aseptic reconstitution with mHTF or other appropriate media prior to use.
As a powder, PVP - Lyophilized has a 2 year shelf life and allows for custom PVP concentrations to suit your laboratory processes. Once reconstituted, lyophilized PVP may be stored for up to 3 weeks. This product does not contain protein supplement.
Quality Control Testing:
• Endotoxin by Limulus Amebocyte Lysate (LAL)
• Sterility by the current USP, CFR and Ph. Eur. Sterility Test
• Sperm Motility Recovery Assay
Also available in:
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Product Inserts
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SDS
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Brochures
Tyrode's Solution - Acidified
Cat. No. 99252
12x5ml
High acidity solution for drilling the zona pellucida during assisted hatching or biopsy procedures
Tyrode's Solution - Acidified facilitates assisted hatching by chemically drilling the zona pellucida. With a pH of 2.1-2.5, this solution's high acidity can break the zona pellucida while avoiding damage to the embryo if applied carefully and in small quantities.
Quality Control Testing:
• Endotoxin by Limulus Amebocyte Lysate (LAL)
• Sterility by the current USP, CFR or Ph. Eur. Sterility Test
• pH
• Osmolality
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Product Inserts
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SDS
Embryo Biopsy Medium
Cat. No. 90103
2x20ml
Remove blastomeres for pre-implantation genetic diagnosis (PGD) from Day 3 cleavage-stage embryos
Embryo Biopsy Medium is for temporary culture and holding of day 3 embryos during biopsy. It is an ideal environment for 4-10 cell embryos that will have blastomeres removed for pre-implantation genetic screening or diagnosis.
Contains:
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HEPES-buffered HTF without Ca2+ and Mg2+
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EDTA as chelating agent
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Human serum albumin (5 mg/mL)
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Gentamicin sulfate (10µg/mL)
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Product Inserts
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SDS
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Product Sheets
Oil for Embryo Culture
Cat. No. 9305
100, 500ml
Oil for Embryo Culture is a sterile light mineral oil intended for use as an overlay when culturing in reduced volumes of media to prevent evaporation, and to protect the media from changes in osmolality and pH.
Standard Packaging Size: 100 mL, 500 mL
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Product Inserts
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Product Sheets
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White Papers and Research Articles
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Brochures
Vit Kit®-Freeze
Cat. No. 90133-SO
Vitrification Freeze Kit is for use in the vitrification of oocytes (MII), pronuclear (PN) zygotes through day 3 cleavage stage embryos and blastocyst stage embryos.
Each kit contains liquid and ready-to-use solutions. It does not require CO2 incubation.
2 Vials of ES (Equilibration Solution, 2 x 1 mL). Contains
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7.5% DMSO
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7.5% ethylene glycol
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20% DSS
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Gentamicin
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In a M-199 HEPES Buffered Medium
2 Vials of VS (Vitrification Solution, 2 x 1 mL). Contains
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Pregnancy results following initiation of a blastocyst vitrification program
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Vitrification and warming of donor oocytes using a closed straw system
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Product Inserts
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SDS
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Product Sheets
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Vitrification Brochure
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Protocols
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Brochures
Vit Kit®-Thaw
Cat. No. 90137-SO
Vitrification Thaw Kit is for use in the thawing of vitrified oocytes (MII), pronuclear (PN) zygotes through day 3 cleavage stage embryos and blastocyst stage embryos.
Vitrification Thaw Kit is for use in the thawing of vitrified oocytes (MII), pronuclear (PN) zygotes through day 3 cleavage stage embryos and blastocyst stage embryos.
Each kit contains liquid and ready-to-use solutions. It does not require CO2 incubation.
4 Vials of TS (Thawing Solution, 4 x 2mL). Contains
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1 M sucrose
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20% DSS
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Gentamicin
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In a M-199 HEPES Buffered Medium
1 Vial of DS (Dilution Solution, 1 x 2mL). Contains
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0.5 M sucrose
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20% DSS
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Gentamicin
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In a M-199 HEPES Buffered Medium
1 Vial of WS (Washing Solution, 1 x 2mL). Contains
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20% DSS
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Gentamicin
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In a M-199 HEPES Buffered Medium.
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Pregnancy results following initiation of a blastocyst vitrification program
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Vitrification and warming of donor oocytes using a closed straw system
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Product Inserts
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SDS
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Product Sheets
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Vitrification Brochure
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Protocols
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Brochures
Freezing Medium - TYB with Glycerol & Gentamicin
Cat. No. 90128
20x5ml, 100ml
Sperm cryopreservation medium containing TEST-yolk buffer to attain high postthaw sperm recovery.
Freezing Medium TYB contains TEST-yolk buffer (TYB) to recover more motile sperm post-thaw than glycerol-only media (Hallak 2000, Nallella 2004). TEST-yolk buffer is a semen extender and cryobuffer that can be paired with glycerol to achieve high sperm motility and viability. The egg yolk is from USDA certified virus-free flocks and washed from the sperm after cryopreservation. Freezing Medium TYB is also the only CE-marked and FDA-approved sperm freezing media containing TYB and glycerol. As one of the most referenced sperm cryopreservation media worldwide, Freezing Medium TYB has established itself as the standard for performance.
• 2 year shelf life
• Contains:
- 20% egg yolk from USDA certified SPF (Virus Free) laying flocks, heat inactivated at 56°C for 30 minutes
- 12% glycerol
- 10µg/mL gentamicin sulfate
• CE Marked
Quality Control Testing:
• Endotoxin by Limulus Amebocyte Lysate (LAL)
• Sperm Cryosurvival assay
• Sterility by the current USP, CFR and Ph. Eur. Sterility Test
• pH
• Osmolality
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Product Inserts
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SDS
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Product Sheets
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Protocols
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Brochures
Sperm Maintenance Medium
Cat. No. 99176
Sperm cryopreservation medium to minimize dilution of low quality semen for sperm freezing and preservation
Sperm Maintenance Medium uses only 1 mL of medium per 3 mL of semen or sperm. This more efficient medium means lower costs per cycle of sperm cryopreservation, minimal dilution of low quality semen, and potentially fewer straws/vials per patient. Sperm Maintenance Medium has similar performance to Freezing Medium TYB, a TEST-yolk buffer based sperm cryopreservation medium, without containing egg yolk (Es-slami, 2009). The medium’s unique formulation contains ingredients, such as hypotaurine and ascorbic acid, that have been shown to protect against reactive oxygen species.
• CE Marked
• Contains:
- 28% v/v glycerol for a 3:1 ratio of semen to medium
- Antioxidants, such as hypotaurine and ascorbic acid
- HEPES
- Human Serum Albumin
- Sucrose
- Pluronic F-68
Quality Control Testing:
• Endotoxin by Limulus Amebocyte Lysate (LAL)
• Sperm Cryosurvival assay
• Sterility by the current USP , CFR or Ph. Eur. Sterility Test
• pH
• Osmolality
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Product Inserts
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SDS
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Product Sheets
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Protocols
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Brochures
Refrigeration Medium - TYB with Gentamicin
Cat. No. 90129
Short term storage of human sperm using an easy single step refrigeration process that preserves semen for up to 4 days without freezing or glycerol
Refrigeration Medium TYB with Gentamicin is ideal for short term sperm storage to preserve sperm for up to 4 days. With an easy one-step process of simply thawing the media and adding media at a 1:1 ratio with the sperm sample, it is quicker and easier than sperm cryopreservation. Refrigeration Medium TYB with Gentamicin contains TEST-yolk buffer as the protecting agent, and the glycerol-free formulation avoids the potentially damaging effects from penetrating cryoprotectants. This product has been CE marked and FDA-cleared for short term sperm storage.
• Contains
- 20% egg yolk - from USDA certified Specific Pathogen Free (Virus Free) laying flocks, heat inactivated at 56° C for 30 minutes
- 10µg/mL Gentamicin Sulfate
- Does not contain glycerol
• CE Mark
Quality Control Testing:
• Endotoxin by Limulus Amebocyte Lysate (LAL)
• Sperm Motility Recovery Assay
• Sterility by the current USP, CFR or Ph. Eur. Sterility Test
• pH
• Osmolality
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Product Inserts
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SDS
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Product Sheets
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Brochures